Blood - FFP - Cryo - Platelets

Quick Guide to Use and Storage of Blood and Blood Products in the
Stanford University Hospital/LPCH Operating Rooms

1. Whole Blood or Packed RBC’s:         $ 114. - $ 174. per unit

Ordering Time

If patient already has blood typed and screened, blood can be sent to the OR within 10 minutes of request.

Important: True emergency (bleeding-out) situations should be clearly communicated to the blood bank early so that they can provide expedited support and uncrossmatched blood if necessary.

Storage

Must be kept in an ice bucket at all times.

Indicator dot changes color when blood warms above 8 degrees C. Blood is then discarded if not used. 

Indications

Rarely indicated with Hb above 10, almost always indicated with Hb below 6.

Allowable Pre-Transfusion Blood Loss:

 V =  EBV x  Ho – Hf
                          Hav

V = Volume to be removed or Blood Loss

EBV = Estimated Blood Volume

Ho  = Initial Hct

Hf   = Desired Hct

Hav = Average Hct (Average of Ho and Hf)

 

EBV:

Premature:      100 ml/kg   

Newborn:         80 ml/kg 

Adult Male:      70 ml/kg 

Adult Female:   65 ml/kg   

                  

Dose/Efficacy

Adults:
1 unit increases Hb by 1 g/dL or Hct by 3-4 %

Pediatrics:
Transfusion of 8 ml/kg increases Hb by 1 g/dL

Recommendation

Type and screen patients  but do not order blood units  until you are fairly certain you intend to transfuse. As long as the patient does not have antibodies (a normal screen), you can get blood within 10 minutes.

Unused blood must be returned to Blood Bank if indicator dot shows that blood has not warmed. Units should be kept in the cooler except when in transit.

Note: Temperature indicators are not used on split-units. Split-units will be discarded if not returned within 30 minutes.

RBC’s must be transfused within 4 hours of issue if not kept in a cooler.


2. Fresh Frozen Plasma (FFP):            $ 75.00 per unit

Ordering Time

Requires minimum of 40 minutes to thaw and label. Available in 10 minutes if already thawed.

Storage

Store in OR at room temperature. 

If left in the blood bank after thawing, FFP can be used on any patient within 24 hours.

When FFP has been out of the blood bank for 30 minutes, it cannot be re-cycled for another patient and is discarded.

Indications

PT or PTT ratio (test value/control value) should be at least 1.5 before FFP is used.

No compatibility testing required, but ABO-compatible FFP should be used. Therefore patient ABO-typing is required.

Dose/Efficacy

Volume of 1 unit is 200-250 ml. Usual adult dose is 4-6 units.

This will increase coagulation factors by 20 % immediately.

Recommendations

Order FFP units to be thawed, but do not actually send for any units until you are sure you intend to give them.

Leave any thawed but un-needed units in the blood bank for re-cycling. (For example, you can ask for 6 units to be thawed and later decide that you only need 2 units. If you leave the remaining 4 units in the blood bank, they are available for any ABO-compatible patient for the next 24 hours.)



3. Cryoprecipitate:                 $ 53.00 per unit,   $706.00 per pooled 10 units

Ordering Time

Requires minimum of 40 minutes to thaw, pool, and label.

Indications

A concentrated form of certain plasma proteins from FFP.

Indicated for fibrinogen, Factor VIII, and Factor XIII deficiency only.
(von Willebrand’s disease patients unresponsive to desmopressin and bleeding patients with fibrinogen levels below 80-100 mg/dL.)

No Factor V contained in cryoprecipitate.

Dose/Efficacy

Delivered as a pooled unit of 10 units.

Contains concentrated Factor VIII (von Willebrand factor), fibrinogen, and Factor XIII.

Each unit increases fibrinogen in adults by 5 mg/dL.

Hemostatic fibrinogen level is > 100 mg/dL.

No compatibility testing, but ABO-compatible cryo should be used when possible.

Recommendations

Order cryoprecipitate units to be thawed only when definitely needed.

Pooled cryoprecipitate expires 4 hours after pooling.

Storage

Store in OR at room temperature

Never refrigerate- factors will precipitate out.

When cryoprecipitate has been out of the blood bank for 30 minutes, it cannot be re-cycled for another patient and is discarded.


4. Platelets:         $ 75.00 per single unit,   $ 603. - $834. per pheresis pack (6 units)

Ordering Time

Available from blood bank within 10 minutes via tube system.

Storage

Store in OR at room temperature

Never refrigerate- if cooled, platelets are inactivated.

Indications

Usually indicated in surgical patients with platelet counts below 50,000/mcl. Rarely indicated with platelet counts above 100,000/mcl.

No compatibility testing, but ABO-compatible platelets should be used when possible.

Dose/Efficacy

Adult dose is 6 units of random platelets or 1 bag of platelet pheresis pack

One dose increases platelet count in adults by 30-60,000/mcl.

Recommendations

Do not order until you are sure that you plan to administer platelets. They will be discarded if returned to blood bank in over 1 hour.


References:

1.  Practice Guidelines for Blood Component Therapy: A Report by the American Society of Anesthesiologists Task Force on Blood Component Therapy. Anesthesiology 1996: 84:732-47 (c) 1996 American Society of Anesthesiologists. Available on the Internet at: (http://www.asahq.org/Practice/Blood/Blood_Component.html)

2.  Blood Transfusion Therapy: A Physician’s Handbook. 5th Edition.  American Association of Blood Banks, 1996.

3. Stehling L. and Zauder H.L., Acute normovolemic hemodilution. Transfusion 31:9, pg. 857-868,  1991.

4. Goodnough LT et al, Transfusion Medicine. NEJM 340:6, 2 parts, 1999.

 

J. Kent Garman, M.D., M.S.
Stanford University Department of Anesthesia